Key Players Research Articles

Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control

Graves' ophthalmopathy (GO) is an extrathyroidal manifestation of Graves' disease, Orbital fibroblasts (OFs) are recognized as key players in GO pathogenesis, involved in orbital inflammation, tissue remodeling, and fibrosis. This study offers a primary exploration of cell behavior and characteristics on OFs from GO (GO-OFs), and compared to OFs from healthy control (HC-OFs). Results reveal that GO-OFs exhibit delayed migration from tissue fragments, while no significant difference in cell proliferation is observed between GO-OFs and HC-OFs. Aberrant expression pattern of surface proteins Thy-1, TSHR, and IGF-1R suggests shared autoantigens and pathways between GO and GD, contributing to inflammation and fibrosis. Investigations into cytokine responses unveil elevated secretion of hyaluronic acid (HA) and prostaglandin E2 (PGE2) in GO-OFs, emphasizing their role in tissue remodeling. These findings deepen our understanding of OFs in GO pathogenesis, offering potential therapeutic avenues.

Prenatal Fluoxetine Exposure Influences Glucocorticoid Receptor-Mediated Activity in the Prefrontal Cortex of Adolescent Rats Exposed to Acute Stress.

Any deviation from the programmed processes of brain development may modify its formation and functions, thereby precipitating pathological conditions, which often become manifest in adulthood. Exposure to a challenge during crucial periods of vulnerability, such as adolescence, may reveal molecular changes preceding behavioral outcomes. Based on a previous study showing that prenatal fluoxetine (FLX) leads to the development of an anhedonic-like behavior in adult rats, we aimed to assess whether the same treatment regimen (i.e., fluoxetine during gestation; 15 mg/kg/day) influences the ability to respond to acute restraint stress (ARS) during adolescence. We subjected the rats to a battery of behavioral tests evaluating the development of various phenotypes (cognitive deficit, anhedonia, and anxiety). Furthermore, we carried out molecular analyses in the plasma and prefrontal cortex, a brain region involved in stress response, and whose functions are commonly altered in neuropsychiatric conditions. Our findings confirm that prenatal manipulation did not affect behavior in adolescent rats but impaired the capability to respond properly to ARS. Indeed, we observed changes in several molecular key players of the hypothalamic pituitary adrenal axis, particularly influencing genomic effects mediated by the glucocorticoid receptor. This study highlights that prenatal FLX exposure influences the ability of adolescent male rats to respond to an acute challenge, thereby altering the functionality of the hypothalamic-pituitary-adrenal axis, and indicates that the prenatal manipulation may prime the response to challenging events during this critical period of life.

Elevated aerobic glycolysis driven by p62-mTOR axis promotes arsenic-induced oncogenic phenotypes in human mammary epithelial cells.

Chronic arsenic exposure is considered to increase the risk of breast cancer. p62 is a multifunctional adaptor protein that controls myriad cellular processes and is overexpressed in breast cancer tissues. Although previous studies have indicated the involvement of p62 accumulation in arsenic tumorigenesis, the underlying mechanism remains obscure. Here, we found that 0.1 µM or 0.5 µM arsenite exposure for 24 weeks induced oncogenic phenotypes in human mammary epithelial cells. Elevated aerobic glycolysis, cell proliferation capacity, and activation of p62-mTOR pathway, as indicated by increased protein levels of p62, phosphorylated-mTOR (p-mTOR) and hypoxia-inducible factor 1α (HIF1α), were observed in chronically arsenite-exposed cells, and of note in advance of the onset of oncogenic phenotypes. Moreover, p62 silencing inhibited acquisition of oncogenic phenotypes in arsenite-exposed cells. The protein levels of p-mTOR and HIF1α, as well as aerobic glycolysis and cell proliferation, were suppressed by p62 knockdown. In addition, re-activation of p‑mTOR reversed the inhibitory effects of p62 knockdown. Collectively, our data suggest that p62 exerts an oncogenic role via mTORC1 activation and acts as a key player in glucose metabolism during arsenite-induced malignant transformation, which provides a new mechanistic clue for the arsenite carcinogenesis.

The role of serotonin in depression-A historical roundup and future directions.

Depression is one of the most common psychiatric disorders worldwide, affecting approximately 280 million people, with probably much higher unrecorded cases. Depression is associated with symptoms such as anhedonia, feelings of hopelessness, sleep disturbances, and even suicidal thoughts. Tragically, more than 700 000 people commit suicide each year. Although depression has been studied for many decades, the exact mechanisms that lead to depression are still unknown, and available treatments only help a fraction of patients. In the late 1960s, the serotonin hypothesis was published, suggesting that serotonin is the key player in depressive disorders. However, this hypothesis is being increasingly doubted as there is evidence for the influence of other neurotransmitters, such as noradrenaline, glutamate, and dopamine, as well as larger systemic causes such as altered activity in the limbic network or inflammatory processes. In this narrative review, we aim to contribute to the ongoing debate on the involvement of serotonin in depression. We will review the evolution of antidepressant treatments, systemic research on depression over the years, and future research applications that will help to bridge the gap between systemic research and neurotransmitter dynamics using biosensors. These new tools in combination with systemic applications, will in the future provide a deeper understanding of the serotonergic dynamics in depression.

Plasma membrane SK2 channel activity regulates migration and chemosensitivity of high-grade serous ovarian cancer cells.

No data are currently available on the functional role of small conductance Ca2+ -activated K+ channels (SKCa) in ovarian cancer. Here, we characterized the role of SK2 (KCa2.2) in ovarian cancer cell migration and chemosensitivity. Using the selective non-cell-permeant SK2 inhibitor Lei-Dab7, we identified functional SK2 channels at the plasma membrane, regulating store-operated Ca2+ entry (SOCE) in both cell lines tested (COV504 and OVCAR3). Silencing KCNN2 with short interfering RNA (siRNA), or blocking SK2 activity with Lei-Dab7, decreased cell migration. The more robust effect of KCNN2 knockdown compared to Lei-Dab7 treatment suggested the involvement of functional intracellular SK2 channels in both cell lines. In cells treated with lysophosphatidic acid (LPA), an ovarian cancer biomarker of progression, SK2 channels are a key player of LPA pro-migratory activity but their role in SOCE is abolished. Concerning chemotherapy, SK2 inhibition increased chemoresistance to Taxol® and low KCNN2 mRNA expression was associated with the worst prognosis for progression-free survival in patients with serous ovarian cancer. The dual roles of SK2 mean that SK2 activators could be used as an adjuvant chemotherapy to potentiate treatment efficacy and SK2 inhibitors could be administrated as monotherapy to limit cancer cell dissemination.

Bulacan Feedmill Plant Towards Business Operations Sustainability Framework

In today’s rapidly changing global landscape, businesses are increasingly recognizing the imperative of incorporating sustainability principles into their operations. As the world faces pressing environmental and social challenges, companies must evolve and adopt responsible practices that not only safeguard the planet but also ensure long-term profitability and resilience. The Bulacan Feedmill Plant, a key player in the animal nutrition industry in the Philippines, is no exception to this paradigm shift. As such, this research delves into the realm of sustainable business operations, with a specific focus on developing a comprehensive Business Operations Sustainability Framework tailored to the unique context of the Bulacan Feed mill Plant. Amidst the growing global discourse on sustainable practices, there is a perceived gap in the literature concerning the application of sustainability principles within the animal nutrition industry, particularly in the context of Feedmill plants. While various frameworks exist for other industries, limited attention has been given to developing tailored sustainability frameworks for the animal nutrition sector. Hence, this research endeavors to bridge this gap and contribute to the body of knowledge by providing a comprehensive and industry-specific roadmap for the Bulacan Feedmill Plant, paving the way for a more sustainable and thriving future. Based on the findings, the following are recommended; 1) Develop a comprehensive Business Operation Sustainable Framework aligned with sustainability principles, including clear goals, strategies, and actionable plans for economic, environmental, and social sustainability. 2) Engage and educate employees through awareness programs, training, and workshops to ensure their understanding of the importance of sustainability and their role in driving positive change within the organization. 3) Actively involve customers in sustainability initiatives by providing information about sustainable products and encouraging them to make environmentally conscious choices. 4) Prioritize sustainable procurement practices by collaborating with suppliers who adhere to sustainable principles to ensure an environmentally responsible and socially conscious supply chain. 5) Engage with local communities, listen to their concerns, and involve them in sustainable development projects to foster mutual understanding and support. Keywords: sustainability framework, business operations, feed mill plant, sustainability strategy

Transkingdom Network Analysis (TkNA): a systems framework for inferring causal factors underlying host-microbiota and other multi-omic interactions.

We present Transkingdom Network Analysis (TkNA), a unique causal-inference analytical framework that offers a holistic view of biological systems by integrating data from multiple cohorts and diverse omics types. TkNA helps to decipher key players and mechanisms governing host-microbiota (or any multi-omic data) interactions in specific conditions or diseases. TkNA reconstructs a network that represents a statistical model capturing the complex relationships between different omics in the biological system. It identifies robust and reproducible patterns of fold change direction and correlation sign across several cohorts to select differential features and their per-group correlations. The framework then uses causality-sensitive metrics, statistical thresholds and topological criteria to determine the final edges forming the transkingdom network. With the subsequent network's topological features, TkNA identifies nodes controlling a given subnetwork or governing communication between kingdoms and/or subnetworks. The computational time for the millions of correlations necessary for network reconstruction in TkNA typically takes only a few minutes, varying with the study design. Unlike most other multi-omics approaches that find only associations, TkNA focuses on establishing causality while accounting for the complex structure of multi-omic data. It achieves this without requiring huge sample sizes. Moreover, the TkNA protocol is user friendly, requiring minimal installation and basic familiarity with Unix. Researchers can access the TkNA software at https://github.com/CAnBioNet/TkNA/ .

Discovery of the sEH Inhibitor Epoxykynin as a Potent Kynurenine Pathway Modulator.

Disease-related phenotypic assays enable unbiased discovery of novel bioactive small molecules and may provide novel insights into physiological systems and unprecedented molecular modes of action (MMOA). Herein, we report the identification and characterization of epoxykynin, a potent inhibitor of the soluble epoxide hydrolase (sEH). Epoxykynin was discovered by means of a cellular assay monitoring modulation of kynurenine (Kyn) levels in BxPC-3 cells upon stimulation with the cytokine interferon-γ (IFN-γ) and subsequent target identification employing affinity-based chemical proteomics. Increased Kyn levels are associated with immune suppression in the tumor microenvironment and, thus, the Kyn pathway and its key player indoleamine 2,3-dioxygenase 1 (IDO1) are appealing targets in immuno-oncology. However, targeting IDO1 directly has led to limited success in clinical investigations, demonstrating that alternative approaches to reduce Kyn levels are in high demand. We uncover a cross-talk between sEH and the Kyn pathway that may provide new opportunities to revert cancer-induced immune tolerance.

An ancient bacterial zinc acquisition system identified from a cyanobacterial exoproteome.

Bacteria have developed fine-tuned responses to cope with potential zinc limitation. The Zur protein is a key player in coordinating this response in most species. Comparative proteomics conducted on the cyanobacterium Anabaena highlighted the more abundant proteins in a zur mutant compared to the wild type. Experimental evidence showed that the exoprotein ZepA mediates zinc uptake. Genomic context of the zepA gene and protein structure prediction provided additional insights on the regulation and putative function of ZepA homologs. Phylogenetic analysis suggests that ZepA represents a primordial system for zinc acquisition that has been conserved for billions of years in a handful of species from distant bacterial lineages. Furthermore, these results show that Zur may have been one of the first regulators of the FUR family to evolve, consistent with the scarcity of zinc in the ecosystems of the Archean eon.

Eosinophil activation during immune responses: an ultrastructural view with an emphasis on viral diseases.

Eosinophils are cells of the innate immune system that orchestrate complex inflammatory responses. The study of the cell biology of eosinophils, particularly associated with cell activation, is of great interest to understand their immune responses. From a morphological perspective, activated eosinophils show ultrastructural signatures that have provided critical insights into the comprehension of their functional capabilities. Application of conventional transmission electron microscopy (TEM) in combination with quantitative assessments (quantitative TEM), molecular imaging (immunoEM), and three-dimensional (3D) electron tomography have generated important insights into mechanisms of eosinophil activation. This review explores a multitude of ultrastructural events taking place in eosinophils activated in vitro and in vivo as key players in allergic and inflammatory diseases, with an emphasis on viral infections. Recent progress in our understanding of biological processes underlying eosinophil activation, including in vivo mitochondrial remodeling, is discussed, and it can bring new thinking to the field.

Correlation between maternal and infant vitamin B12 levels in severe malnutrition in infants under 6months.

Malnutrition poses a significant global health challenge, affecting various age groups, with infants under 6months being particularly vulnerable. Vitamin B12, an essential micronutrient critical for neurological development, has been identified as a key player in the overall health of both mothers and infants. To find the correlation between serum vitamin B12 levels in infants, 1-6months of age with severe malnutrition and maternal levels at tertiary care hospitals in western Rajasthan. The cross-sectional study was conducted in the UNICEF Regional Center of Excellence-supported Nutrition Rehabilitation Center in Rajasthan, India, through simple random sampling. One hundred ten infants with their mothers were enrolled after consent and approval from the Institutional Ethics Committee. Severe malnutrition predominantly affected infants aged 1-2months, with 77% born small for gestational age and 66.4% belonging to the multiple birth order group. Serum vitamin B12 levels showed a significant positive correlation between mothers and infants (p < 0.001), while exclusive breastfeeding correlated positively with age-appropriate milestones (p = 0.033). The findings emphasize the importance of targeted interventions addressing maternal and infant nutrition, with a focus on ensuring adequate vitamin B12 levels.

Computer-aided drug discovery of c-Abl kinase inhibitors from plant compounds against chronic myeloid leukemia

Chronic myeloid leukemia (CML) is a hematological malignancy characterized by the neoplastic transformation of hematopoietic stem cells, driven by the Philadelphia (Ph) chromosome resulting from a translocation between chromosomes 9 and 22. This Ph chromosome harbors the breakpoint cluster region (BCR) and the Abelson (ABL) oncogene (BCR-ABL1) which have a constitutive tyrosine kinase activity. However, the tyrosine kinase activity of BCR-ABL1 have been identified as a key player in CML initiation and maintenance through c-Abl kinase. Despite advancements in tyrosine kinase inhibitors, challenges such as efficacy, safety concerns, and recurring drug resistance persist. This study aims to discover potential c-Abl kinase inhibitors from plant compounds with anti-leukemic properties, employing drug-likeness assessment, molecular docking, in silico pharmacokinetics (ADMET) screening, density function theory (DFT), and molecular dynamics simulations (MDS). Out of 58 screened compounds for drug-likeness, 44 were docked against c-Abl kinase. The top hit compound (isovitexin) and nilotinib (control drug) were subjected to rigorous analyses, including ADMET profiling, DFT evaluation, and MDS for 100 ns. Isovitexin demonstrated a notable binding affinity (-15.492 kcal/mol), closely comparable to nilotinib (-16.826 kcal/mol), showcasing a similar binding pose and superior structural stability and reactivity. While these findings suggest isovitexin as a potential c-Abl kinase inhibitor, further validation through urgent in vitro and in vivo experiments is imperative. This research holds promise for providing an alternative avenue to address existing CML treatment and management challenges. Communicated by Ramaswamy H. Sarma

Interrogating endothelial barrier regulation by temporally resolved kinase network generation.

Kinases are key players in endothelial barrier regulation, yet their temporal function and regulatory phosphosignaling networks are incompletely understood. We developed a novel methodology, Temporally REsolved KInase Network Generation (TREKING), which combines a 28-kinase inhibitor screen with machine learning and network reconstruction to build time-resolved, functional phosphosignaling networks. We demonstrated the utility of TREKING for identifying pathways mediating barrier integrity after activation by thrombin with or without TNF preconditioning in brain endothelial cells. TREKING predicted over 100 kinases involved in barrier regulation and discerned complex condition-specific pathways. For instance, the MAPK-activated protein kinase 2 (MAPKAPK2/MK2) had early barrier-weakening activity in both inflammatory conditions but late barrier-strengthening activity exclusively with thrombin alone. Using temporal Western blotting, we confirmed that MAPKAPK2/MK2 was differentially phosphorylated under the two inflammatory conditions. We further showed with lentivirus-mediated knockdown of MAPK14/p38α and drug targeting the MAPK14/p38α-MAPKAPK2/MK2 complex that a MAP3K20/ZAK-MAPK14/p38α axis controlled the late activation of MAPKAPK2/MK2 in the thrombin-alone condition. Beyond the MAPKAPK2/MK2 switch, TREKING predicts extensive interconnected networks that control endothelial barrier dynamics.

Understanding Consumer Adaptations in Response to COVID-19: A Rural Canadian Perspective

ABSTRACT Food system disruptions impact consumer purchasing patterns. A cross-sectional survey to determine priorities and factors influencing shopping and food procurement habits due to COVID-19 was promoted through social media. We recruited a convenience sample (n = 334) using Qualtrics in Nova Scotia, Canada. Descriptive statistics described age, gender, and income levels. Ordinal regression assessed predictors of shopping pre-pandemic. Likert scales were analyzed using the related samples marginal homogeneity test. Chi-squared tested relationships between income and the amount spent on food. Consumers demonstrated their willingness to support local producers and may be key players in constructing resilient local food systems.

Technological Capabilities of A Higher Education Institution in Quezon Province: Basis for Organizational Competitiveness Framework

Technological advancement is consistently seen developing and evolving in such a fast phase which consequently brings challenges to a number of key players in the industry including that of the academe and higher education institutions – for which the same are thrusted to deliver quality education. With the ever-increasing development of technology and its integration into an organization’s systems and processes, a decision regarding its implementation and use is a challenge, more so on capabilities and opportunities brought about by these phenomena. One important consideration for a globally competitive higher education provider is technological transformation. This study dwelled on the technological capabilities of a Philippine higher education institution (HEI) located in the province of Quezon. A total of three hundred three (n=303) individuals consented to participate in the study. Furthermore, the imperatives on how higher education institutions can self-develop towards technological capacitation were explored, to support its mission and vision, and continue its delivery of teaching-learning amidst the volatility, uncertainty, complexity, and ambiguity of the education industry. Specifically, the dimensions of information and communication technology infrastructure, technology adoption, human capital, investment innovation in technology, and innovative technology environment were conceptualized as basic tenets of technological transformation. The impact of these dimensions on organizational competitiveness was measured using partial-least square structural equation modeling (PLS-SEM). The practical implications of this study are to contribute to the theory and practice of technology in education and fill the gaps in the limited literature resources on technology transformation in education, particularly in the province of Quezon. Keywords: technological capability, organizational competitiveness, higher education institution, Quezon province

Revitalizing Meisen Kimono in Chichibu through Storytelling—Craft, Community, and Sense-Making

Japan’s kimono has seen a gradual revitalization since the 2000s. Especially the renewed interest in antique and meisen kimono has been of major importance. Responding to this, meisen creators in Chichibu have actively started processes of meisen’s reevaluation and re-interpretation through storytelling. Research focusing on the activities and storytelling by Chichibu’s weavers and craftspeople in revitalizing meisen has remained limited, and therefore this research focuses on a case study of weaving studio Arakei Orimono, together with other key players who helped develop and sustain the popularity of meisen in the area. This paper takes up a content and narrative analysis conducted from the framework of storytelling and meaning-making, making use of material gathered through observations, newspapers, magazine articles, testimonials, websites, blogs, Facebook, Instagram, and interviews. The interviews in this research were conducted using an oral history approach. This paper argues that the usage of stories to analyze processes of making has led the meisen craftspeople in Chichibu to develop new insights into how to understand and redefine their craft in new ways, allowing them to reflect on their making processes and the meaning of their activities, contributing to the further revitalization of meisen.

Blocking Ubiquitin-Specific Protease 7 Induces Ferroptosis in Gastric Cancer via Targeting Stearoyl-CoA Desaturase.

Gastric cancer (GC) presents a formidable global health challenge, and conventional therapies face efficacy limitations. Ubiquitin-specific protease 7 (USP7) plays pivotal roles in GC development, immune response, and chemo-resistance, making it a promising target. Various USP7 inhibitors have shown selectivity and efficacy in preclinical studies. However, the mechanistic role of USP7 has not been fully elucidated, and currently, no USP7 inhibitors have been approved for clinical use. In this study, DHPO is identified as a potent USP7 inhibitor for GC treatment through in silico screening. DHPO demonstrates significant anti-tumor activity in vitro, inhibiting cell viability and clonogenic ability, and preventing tumor migration and invasion. In vivo studies using orthotopic gastric tumor mouse models validate DHPO's efficacy in suppressing tumor growth and metastasis without significant toxicity. Mechanistically, DHPO inhibition triggers ferroptosis, evidenced by mitochondrial alterations, lipid Reactive Oxygen Species (ROS), Malondialdehyde (MDA) accumulation, and iron overload. Further investigations unveil USP7's regulation of Stearoyl-CoA Desaturase (SCD) through deubiquitination, linking USP7 inhibition to SCD degradation and ferroptosis induction. Overall, this study identifies USP7 as a key player in ferroptosis of GC, elucidates DHPO's inhibitory mechanisms, and highlights its potential for GC treatment by inducing ferroptosis through SCD regulation.

From Green Vision to Market Reality: Exploring Sustainable Initiatives and Challenges in India

In the era of globalization and heightened environmental awareness, the relevance of green marketing has surged in India. This exploratory study investigates the evolution and significance of green marketing, shedding light on its golden rules and integration into the marketing mix. Emphasizing the imperative for sustainable green marketing, the study explores its objectives and delves into challenges faced by Indian companies. Recent data underscores the profitability of green marketing, propelled by consumer demand. A scrutiny of the Indian corporate sector reveals noteworthy green initiatives by key players such as H.C.L., O.N.G.C., Kansai Nerolac, Wipro, I.T.C., Tata, and Godrej. Challenges encountered include conveying new environmental concepts, managing costs, and gaining stakeholder cooperation. The study advocates aligning marketing practices with environmental considerations for holistic, eco-friendly approaches fostering sustainable growth in both business and the environment.

Tailoring Zinc Ferrite Nanoparticle Surface Coating for Macrophage-Affinity Magnetic Resonance Imaging of Atherosclerosis.

Atherosclerosis is a chronic inflammatory disease characterized by the formation of atherosclerotic plaques, while macrophages as key players in plaque progression and destabilization are promising targets for atherosclerotic plaque imaging. Contrast-enhanced magnetic resonance imaging (CE-MRI) has emerged as a powerful noninvasive imaging technique for the evaluation of atherosclerotic plaques within arterial walls. However, the visualization of macrophages within atherosclerotic plaques presents considerable challenges due to the intricate pathophysiology of the disease and the dynamic behavior of these cells. Biocompatible ferrite nanoparticles with diverse surface ligands possess the potential to exhibit distinct relaxivity and cellular affinity, enabling improved imaging capabilities for macrophages in atherosclerosis. In this work, we report macrophage-affinity nanoparticles for magnetic resonance imaging (MRI) of atherosclerosis via tailoring nanoparticle surface coating. The ultrasmall zinc ferrite nanoparticles (Zn0.4Fe2.6O4) as T1 contrast agents were synthesized and modified with dopamine, 3,4-dihydroxyhydrocinnamic acid, and phosphorylated polyethylene glycol to adjust their surface charges to be positively, negatively, and neutrally charged, respectively. In vitro MRI evaluation shows that the T1 relaxivity for different surface charged Zn0.4Fe2.6O4 nanoparticles was three higher than that of the clinically used Gd-DTPA. Furthermore, in vivo atherosclerotic plaque MR imaging indicates that positively charged Zn0.4Fe2.6O4 showed superior MRI efficacy on carotid atherosclerosis than the other two, which is ascribed to high affinity to macrophages of positively charged nanoparticles. This work provides improved diagnostic capability and a better understanding of the molecular imaging of atherosclerosis.

Professional soccer practitioners' perceptions of using performance analysis technology to monitor technical and tactical player characteristics within an academy environment: A category 1 club case study.

This study aimed to identify professional soccer practitioners' perceptions of the application of performance analysis technology within a single academy club. Secondary aims were to understand the importance that practitioners place on monitoring technical and tactical player characteristics, current practices, and barriers to implementing wearable technology. Utilising a mixed method design, forty-four professional soccer academy practitioners (Age = 32 ± 5.8; Years of experience = 8.5 ± 6.2) completed an online survey intended to examine present trends, professional practices, and perceptions regarding the monitoring of technical and tactical metrics. Frequency and percentages of responses for individual items were calculated. Subsequently, eleven participants who were directly involved with the monitoring of players were recruited to participate in a semi-structured interview. Interview data was transcribed and analysed using a combination of deductive and inductive approaches to identify key themes. The main findings across both phases of the study were that (1) technical and tactical metrics are monitored more frequently in matches (Technical: 89%; tactical: 91%) than training (Technical: 80%; Tactical 64%), predominantly due to time constraints and staffing numbers. Accordingly, practitioners believe that it would be beneficial to have an automated way of tracking technical (79%) and tactical (71%) metrics and would consider using a foot-mounted IMU to do so (technical (68%) and tactical (57%)). (2) Monitoring technical and tactical metrics is beneficial to assist with player development and to enrich feedback provision (3) Key stake holders, coaches and players should be informed of the relevance and rationale for monitoring. (4) For successful implementation and continued uptake, the information delivered needs to be both meaningful and easy to understand. Findings suggest that although participants appreciate the need to collect technical and tactical metrics, they are keen to ensure that wearable-derived data does not replace experiential and contextual knowledge. Accordingly, practitioners need to work closely with coaches to determine the contexts in which metrics may or may not prove useful. However, as the sample comprised of participants from a single academy, further studies including more practitioners are warranted. Likewise, future research could also extend to include academy soccer players perceptions too.